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ADAS has again achieved a 100% score in GCN eDNA proficiency testing for 2020!

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ADAS has again correctly identified 100% of blinded eDNA samples in 2020 GCN eDNA proficiency test, this year one of 5 labs to do so in this current round of testing.

ADAS has again achieved a 100% score in GCN eDNA proficiency testing for 2020!

The proficiency testing scheme has been developed to provide evidence of the level of quality in the service offered by the different eDNA testing labs. For underperforming labs, this provides them with an opportunity to recognise and address any problems with their procedures so that they can improve their service. For ecologists using eDNA as a survey tool, it allows them to compare the services offered by each testing lab and the opportunity to discuss with their eDNA analysis supplier how they performed.

2020 Proficiency test summary

Lab A (Negative not inhibited) B
(Low positive not inhibited)
C (Negative not inhibited) D (Inhibited) E
(Low positive not inhibited)
F
(Negative not inhibited)
G (Negative not inhibited) H
(Low positive not inhibited)
I
(High positive not inhibited)
J
(Negative not inhibited)
% Correct
ADAS 0/12 n 3/12 n 0/12 n 0/12 i 2/12 n 0/12 n 0/12 n 2/12 n  12/12 n 0/12 n 100
2 0/12 n 1/12 n 0/12 n 0/12 i 2/12 n 0/12 n 0/12 n 3/12 n  12/12 n 0/12 n 100
3 0/12 n 2/12 n 0/12 n 0/12 i 1/12 n 0/12 n 0/12 n 2/12 n  12/12 n 0/12 n 100
4 0/12 n 4/12 n 0/12 n 0/12 i 2/12 n 0/12 n 0/12 n 2/12 n  12/12 n 0/12 n 100
5 0/12 i 0/12 i 0/12 i 0/12 i 1/12 i 0/12 n 0/12 n 0/12 i 12/12 n 0/12 n 50
6 0/12 n 1/12 n 0/12 n 0/12 n 3/12 n 0/12 n 0/12 n 0/12 i 12/12 n 0/12 n 80
7 0/12 n 5/12 n 0/12 n 0/12 i 3/12 n 0/12 n 0/12 n 3/12 n  12/12 n 0/12 n 100
8 1/12 n 0/12 n 0/12 n 0/12 i 1/12 n 0/12 n 0/12 n 2/12 n  12/12 n 0/12 n 80

 

n: Sample recorded as not inhibited
i: Sample recorded as inhibited
Not Satisfactory result

The results of the 2020 eDNA GCN proficiency tests are presented in the table above. This latest round comprised ten different unknown samples to analyse, labs provided their own eDNA kits so that any level of sample degradation/recovery could also be monitored. This year, 5 of 8 labs correctly identified all samples (compared to 2 of 8 labs in 2019 and 5 of 9 labs spring 2018). One laboratory reported false-positive detection in a negative sample and 3 laboratories missed samples containing low GCN DNA. FAPAS, who oversee the proficiency testing scheme, score results as ‘satisfactory’ if both the PCR result is correct and whether inhibition or not is recorded as expected.


The table shows the full results of the test. Highlighted is the ADAS eDNA lab service (Lab 1 in this test iteration), who again identified all samples correctly. Labs reporting GCN analysis results as ‘not satisfactory’ are highlighted in red. We do not know the identity of the other laboratories taking part.

Find out more

To learn more about our service visit our eDNA service page or email eDNA@adas.co.uk. To avoid disappointment book your analysis slot as soon as possible.

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ADAS is part of the RSK Group. RSK is the UK’s largest privately-owned multi-disciplinary environmental consultancy and one of the fastest-growing companies of its kind in Europe. With operations in Europe, Africa, and the Middle East, our solutions-led consultancy services help organisations conduct business in a compliant, and environmentally-responsible manner

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ADAS is the UK’s largest independent provider of agricultural and environmental consultancy, rural development services and policy advice. We have a unique combination of practical experience, underpinned by science-based data, which allows us to meet the needs of our clients. Our great strength is our breadth and depth of expertise spanning the entire environmental sector.

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